Biochemical Characterization & Validation of the Yeast Saccharomyces Cerevisiaeas
as Model System for the Function of Human Protein Tau
Acta Biomedica Lovaniensia, No. 364

By Tom Vanderbroek
September 2006
Leuven University Press
Ph.D. Dissertation
ISBN: 9058675432
134 pages, Illustrated, 6 1/8 x 9 3/8"


Table of contents
List of abbreviations

Chapter 1. Overview of the literature

1.1. Protein tau
1.2. Yeast as an experimental model for tauopathy

Chapter 2. Materials and methods

2.1. Biochemical characterization of tau in yeast
2.2. Aggregation of tau in vitro

Chapter 3. Identification and isolation of hyperphosphorylated conformationally changed intermediate of human protein tau expressed in yeast

3.1. Introduction
3.2. Results
3.3. Discussion

Chapter 4. Enhanced conformational changes and aggregation of different FTDP-17 mutants with differential contribution of C-terminal phosphorylation

4.1. Introduction
4.2. Results
4.3. Discussion

Chapter 5. Microtubule-binding of recombinant human tau-4R and tau-P301L isolated from yeast deficient in orthologues of GSK-3ß and cdk5

5.1. Introduction
5.2. Results
5.3. Discussion

General discussion
List of publications


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