Biochemical & Immuno-Histochemical Analysis of APP-Processing
& Amyloid Pathology in Single & Multiple Transgenic Mice
as Models for Alzheimer's Disease
Acta Biomedica Lovaniensia, No. 365

By Tom van Dooren
September 2006
Leuven University Press
Ph.D. Dissertation
ISBN: 9058675440
192 pages, Illustrated, 6 1/8 x 9 3/8"


Chapter 1. General introduction

1.1. Alzheimer’s disease (AD)
1.2. Amyloid Precursor Protein (APP)
1.3. Transgenic mice
1.4. BACE
1.5. Apolipoprotein E
1.6. Clearance of Aß
1.7. Therapeutic approaches for Alzheimer’s disease

Chapter 2. Rationale and aims

Chapter 3. Experimental procedures

3.1. Overview of used transgenic mice
3.2. Biochemical analysis of APP-processing in transgenic mouse brain
3.3. Analysis of APP domain structure and identification of a neuron-specific protein in mouse brain by cross-reaction with Mab Alz90
3.4. Pre-clinical evaluation of Nanobodies against amyloid
3.5. Analysis of APP x BACE1 double transgenic mice
3.6. Analysis of APP x PS1 x apoe4 multiple transgenic mice

Chapter 4. Structure-function analysis of APP and its metabolites

4.1. Biochemical analysis of APP-processing
4.2. Characterization of domain structure of human APP identifies a neuron-specific protein in mouse brain by cross-reaction with antibody Alz90
4.3. Pre-clinical evaluation of llama Nanobodies against amyloid

Chapter 5. BACE1 increases amyloid deposition in brain parenchyma but reduces cerebrovascular amyloid angiopathy in aging BACE1 x APP(V717I) double transgenic mice

Chapter 6. Neuronal or glial expression of human ApoE4 affects parenchymal and vascular amyloid pathology differentially in different brain regions of double and triple transgenic mice

Chapter 7. Mechanisms of PS1 and ApoE4 mediated amyloid pathology

Curriculum Vitae

Medical Science

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