Axon Growth, Injury & Regeneration in the Fly Brain
Acta Biomedica Lovaniensia 374
By Maarten Leyssen
December 2006
Institute of Leuven University Press
Distributed by
ISBN: 9058675688
128 pages, 6 x 9½"
OUT OF PRINT
Summary
Samenvatting
Abbreviations
Chapter I: Introduction
I.1. Neuronal networks and behaviour
I.2. Function and dysfunction of the Amyloid Precursor Protein
I.3. Traumatic injury to neurite tracts
I.4. A link between Alzheimer’s disease, APP and traumatic brain injury
I.5. Drosophila melanogaster
Chapter II: Aims
II.1. How does APP affect axonal outgrowth in the Drosophila brain?
II.2. Is there a link between APPL and brain injury in Drosophila?
II.3. A Drosophila model for neurite injury?
Chapter III: Experimental approach, materials and methods
III.1. Drosophila lines and fly husbandry
III.2. The LNv: a model Drosophila CNS neuron
III.3. Genetic manipulation of the sLNv neurons
III.4. Immunohistochemistry
III.5. Detection and quantification of axonal outgrowth phenotypes in the sLNv
III.6. Traumatic brain injury using insect pins
III.7. GST pulldown of AICD with APLIP1
III.8. Culturing whole brain explants on culture plate inserts
III.9. Imaging living cultured brains
III.10. Electrophysiological recordings of LNv in cultured brains
III.11. Injury of sLNv axonal tracts in cultured brains
III.12. Microscopy and image processing
Chapter IV: Results 1: APP affects axonal outgrowth in the Drosophila brain
IV.1. APP promotes axonal arborisation of the small lateral neurons ventral in the Drosophila brain
IV.2. APP induces axonal arborisation post-developmentally in a quantifiable, robust and dose dependent way
IV.3. The C-terminal domain of APP mediates its effects on axonal arborisation
IV.4. APP interacts with the Abl tyrosine kinase signalling pathway to induce axonal arborisation
Chapter V: Results 2: A link between APPL and brain injury in Drosophila
V.1. APPL expression is upregulated after traumatic brain injury in Drosophila
V.2. JNK signalling is activated after traumatic brain injury and is required for APP to induce axonal arborisation
Chapter VI: Results 3: A Drosophila model for neurite injury
VI.1. The lateral neurons ventral: a model for axonal tract injury in Drosophila brains
VI.2. Drosophila whole brain explant and culture on culture plate inserts
VI.3. sLNv axons remain intact in cultured Drosophila brains
VI.4. Functional characteristics of the LNv are maintained in cultured brains
VI.5. Severing of the sLNv axonal bundle
VI.6. Response to injury of the sLNv axonal bundle
VI.7. Axonal outgrowth and growth cone formation in larval brain explants
Chapter VII: Discussion and general conclusions
VII.1. APP affects axonal outgrowth in the Drosophila brain via Abl signalling
VII.2. A link between APPL and brain injury in Drosophila
VII.3. A Drosophila model for neurite injury
References
Curriculum vitae
Acknowledgements
Pulmonary & Thoracic Medicine
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