Rearrangement of EWSR1 & TAF15
with the Transcription Factor
CIZ/NMP4 in Two Novel Recurrent
Translocations t(12;17)(p13;q11) &
t(12;22)(p13;q12) in Acute Leukemia

By Alessandra Martini
September 2004
Leuven University Press
ISBN: 9058673944
117 pages, Illustrated, 6 " x 9 "
$72.50 Paper Original


This is a Ph.D. dissertation. Contents include: General introduction, Hematological malignancies, TAF15, EWSR1, TLS/FUS (TET): a family of putative RNA binding proteins, TET-transcription factors fusions in tumor formation, CIZ/NMP4: a novel putative zinc-finger transcription factor, Rationale and aims, Materials and methods, Polymerase chain reactions (PCR), Sequence analysis, RNA isolation, Reverse transcription-PCR (RT-PCR), Fluorescent In Situ Hybridization (FISH), Western blot, Patient material, Physical mapping, Rapid amplification of cDNA 5' End (5'RACE-PCR), Cloning of the human CIZ, TAF15 and EWSR1, Expression constructs, Electromobility shift assay, Luciferase assay, Cell cultures and transfection, Viral production and transduction, Focus formation assay, Production of Ba/F3-tet-OFF cell lines, Microarray expression profiling, Quantitative real time PCR (qRT-PCR), List of oligonucleotides, Results, Identification of the CIZ gene on chromosome 12p13 and cloning of the CIZ fusions in novel cases of acute leukemia carrying translocation t(12;17)(p13;q11) and t(12;22)(p13;q11) and t(12;22)(p13;q12), Transactivation properties of wild type CIZ and the chimeric proteins, General discussion and perspectives, Summary.

Oncology
Acta Biomedica Lovaniensia No. 320

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